low stringency wash buffer (Thermo Fisher)
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low stringency wash buffer
Low Stringency Wash Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/low+stringency+wash+buffer/pmc11109757-171-10-15
Average 86 stars, based on 1 article reviews
Low Stringency Wash Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/low+stringency+wash+buffer/pmc11109757-171-10-15
Average 86 stars, based on 1 article reviews
low stringency wash buffer - by Bioz Stars,
2026-09
86/100 stars
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Membrane:Article Title: Bacterial virulence factors and uses thereof Article Snippet: The DNA was cross-linked to the membrane by exposing the membrane to UV light for 2 min, followed by 30 min of baking at 80° C. The membrane was prehybridized by washing it in 10 ml of ULTRAhyb Ultrasensitive Hybridization Buffer (Ambion) at 42° C. for 30 min. .. Ten microliters of the prepared probe were then added to the prehybridized membrane in buffer and the probe was hybridized to the membrane overnight at 42° C. Membrane was washed 2 times 5 min in low Article Title: Bacterial virulence factors and uses thereof Article Snippet: The DNA was cross-linked to the membrane by exposing the membrane to UV light for 2 min, followed by 30 min of baking at 80° C. The membrane was prehybridized by washing it in 10 ml of ULTRAhyb Ultrasensitive Hybridization Buffer (Ambion) at 42° C. for 30 min. .. Ten microliters of the prepared probe were then added to the prehybridized membrane in buffer and the probe was hybridized to the membrane overnight at 42° C. Membrane was washed 2 times 5 min in low Incubation:Article Title: Jaagsiekte Sheep Retrovirus Encodes a Regulatory Factor, Rej, Required for Synthesis of Gag Protein Article Snippet: Briefly, RNA (7 g) was denatured in glyoxal-dimethyl sulfoxide loading dye (Ambion, Austin, TX) at 50°C for 30 min, subjected to agarose gel (0.9%) electrophoresis, and transferred to nylon membranes (Hybond-N Plus; GE Healthcare) using transfer buffer (Ambion) as recommended by the manufacturer. .. Membranes were incubated with rotation in hybridization buffer (GE Healthcare) at 42°C for at least 30 min followed by the addition of 32P-labeled JSRV gag- or mammalian GAPDH-specific probes (5 105 cpm/ml; carried in 4 mg salmon sperm DNA) and incubated at 58°C for 12 to 24 h. Membranes were subsequently washed two times in high-stringency wash buffer (300 mM NaCl, 30 mM sodium citrate, 0.1% SDS) at room temperature for 10 min and then washed two times in Hybridization:Article Title: Jaagsiekte Sheep Retrovirus Encodes a Regulatory Factor, Rej, Required for Synthesis of Gag Protein Article Snippet: Briefly, RNA (7 g) was denatured in glyoxal-dimethyl sulfoxide loading dye (Ambion, Austin, TX) at 50°C for 30 min, subjected to agarose gel (0.9%) electrophoresis, and transferred to nylon membranes (Hybond-N Plus; GE Healthcare) using transfer buffer (Ambion) as recommended by the manufacturer. .. Membranes were incubated with rotation in hybridization buffer (GE Healthcare) at 42°C for at least 30 min followed by the addition of 32P-labeled JSRV gag- or mammalian GAPDH-specific probes (5 105 cpm/ml; carried in 4 mg salmon sperm DNA) and incubated at 58°C for 12 to 24 h. Membranes were subsequently washed two times in high-stringency wash buffer (300 mM NaCl, 30 mM sodium citrate, 0.1% SDS) at room temperature for 10 min and then washed two times in Software:Article Title: Jaagsiekte Sheep Retrovirus Encodes a Regulatory Factor, Rej, Required for Synthesis of Gag Protein Article Snippet: Briefly, RNA (7 g) was denatured in glyoxal-dimethyl sulfoxide loading dye (Ambion, Austin, TX) at 50°C for 30 min, subjected to agarose gel (0.9%) electrophoresis, and transferred to nylon membranes (Hybond-N Plus; GE Healthcare) using transfer buffer (Ambion) as recommended by the manufacturer. .. Membranes were incubated with rotation in hybridization buffer (GE Healthcare) at 42°C for at least 30 min followed by the addition of 32P-labeled JSRV gag- or mammalian GAPDH-specific probes (5 105 cpm/ml; carried in 4 mg salmon sperm DNA) and incubated at 58°C for 12 to 24 h. Membranes were subsequently washed two times in high-stringency wash buffer (300 mM NaCl, 30 mM sodium citrate, 0.1% SDS) at room temperature for 10 min and then washed two times in Control:Article Title: IGHMBP2 deletion suppresses translation and activates the integrated stress response Article Snippet: Membranes were pre-hybridized with pre-warmed ULTRAhyb ultrasensitive hybridization buffer (AM8670; Invitrogen ) at 52°C for 1 h, followed by addition of 10 pmol of each probe. .. The membranes were hybridized overnight and washed two times with Labeling:Article Title: IGHMBP2 deletion suppresses translation and activates the integrated stress response Article Snippet: Membranes were pre-hybridized with pre-warmed ULTRAhyb ultrasensitive hybridization buffer (AM8670; Invitrogen ) at 52°C for 1 h, followed by addition of 10 pmol of each probe. .. The membranes were hybridized overnight and washed two times with |
